cell cycle detection kits annexin v fitc pi  (Beijing Solarbio Science)

Journal: Frontiers in Oncology

Article Title: CIK-augmented anti-PD1/CTLA4 immunotherapy eradicates chemo-resistant ovarian cancer via tripartite mechanistic synergy

doi: 10.3389/fonc.2025.1670033

Figure Lengend Snippet: Treatment effects on ovarian cancer cell apoptosis. (A-F) Annexin V-FITC/PI flow cytometry scatter plots of (A/C/E) A2780 and (B/D/F) SKOV3 cells under control, N+I, and N+I+CIK treatments, respectively. (G/H) Corresponding quantitative analysis of apoptosis rates for (G) A2780 and (H) SKOV3 cells under the indicated treatments. n=3, ***=0.0009, ***=0.0001, ***=0.0001, ***=0.0001 vs respective control groups.

Article Snippet: Immune checkpoint inhibitors Nivolumab (HY-P9903, MCE) and Ipilimumab (HY-P9901, MCE); apoptosis and cell cycle detection kits Annexin V-FITC/PI (CA1020, Solarbio) and DNA Content Assay Kit (CA1510, Solarbio); extracellular matrix component Matrigel (356234, Corning); cell viability reagent CCK-8 Kit (CK04, Dojindo); staining agent Crystal Violet (C8470, Solarbio); and fixation solution 4% Paraformaldehyde (G1113, Servicebio).

Techniques: Flow Cytometry, Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: HDAC3 deteriorates colorectal cancer progression via microRNA-296-3p/TGIF1/TGFβ axis

doi: 10.1186/s13046-020-01720-w

Figure Lengend Snippet: Knockdown of HDAC3 inhibits CRC cell proliferation and metastasis. a . RT-qPCR detection of HDAC3 mRNA, miR-296-3p and TGIF1 mRNA expression in cells after knockdown of HDAC3; b . Western blot analysis determination of HDAC3 and TGIF1 protein expression in cells after knockdown of HDAC3; c . Western blot analysis determination of TGFβ signaling pathway-related proteins in cells after knockdown of HDAC3; d . MTT assay of cell viability in cells after knockdown of HDAC3; e . EdU assay of cell proliferation after knockdown of HDAC3; f . Flow cytometry of cell cycle distribution after knockdown of HDAC3; g . Flow cytometry of cell apoptosis after knockdown of HDAC3; h . Scratch test of cell migration after knockdown of HDAC3; i . Transwell assay of cell invasion after knockdown of HDAC3; j . RT-qPCR detection of PCNA, ki-67, MMP2 and MMP9 expression in cells after knockdown of HDAC3. N = 3. One-way ANOVA was utilized for comparisons among multiple groups, followed by Tukey’s multiple comparisons test. * represented P < 0.05, ** represented P < 0.01, *** represented P < 0.001. si-NC; HDAC3 siRNA negative control; si-HDAC3; siRNA HDAC3

Article Snippet: Cell cycle and Annexin V-FITC/PI apoptosis detection kits were entrusted to BD Biosciences (Franklin Lakes, NJ, USA).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, MTT Assay, EdU Assay, Flow Cytometry, Migration, Transwell Assay, Negative Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: HDAC3 deteriorates colorectal cancer progression via microRNA-296-3p/TGIF1/TGFβ axis

doi: 10.1186/s13046-020-01720-w

Figure Lengend Snippet: Restored miR-296-3p or depleted TGIF1 suppresses CRC cell proliferation and metastasis. a . RT-qPCR detection of miR-296-3p and TGIF1 mRNA expression in cells after up-regulating miR-296-3p or down-regulating TGIF1; b . Protein bands of TGIF1 protein in cells after up-regulating miR-296-3p or down-regulating TGIF1; c . Comparison of TGIF1 protein expression in cells after up-regulating miR-296-3p or down-regulating TGIF1; d . Analysis of TGFβ signaling pathway-related proteins in cells after up-regulating miR-296-3p or down-regulating TGIF1; e . MTT assay of cell viability after up-regulating miR-296-3p or down-regulating TGIF1; f . EdU assay of cell proliferation after up-regulating miR-296-3p or down-regulating TGIF1; g . Flow cytometry of cell cycle distribution after up-regulating miR-296-3p or down-regulating TGIF1; h . Flow cytometry of cell apoptosis after up-regulating miR-296-3p or down-regulating TGIF1; i . Scratch test of cell migration after up-regulating miR-296-3p or down-regulating TGIF1; j . Transwell assay of cell invasion after up-regulating miR-296-3p or down-regulating TGIF1; k . RT-qPCR of PCNA, ki-67, MMP2 and MMP9 expression after up-regulating miR-296-3p or down-regulating TGIF1. N = 3. One-way ANOVA was utilized for comparisons among multiple groups, followed by Tukey’s multiple comparisons test. * represented P < 0.05, ** represented P < 0.01, *** represented P < 0.001. si-CTR; TGIF1 siRNA negative contro; si-TGIF1: siRNA TGIF1; oe-TGIF1: TGIF1 overexpression vector

Article Snippet: Cell cycle and Annexin V-FITC/PI apoptosis detection kits were entrusted to BD Biosciences (Franklin Lakes, NJ, USA).

Techniques: Quantitative RT-PCR, Expressing, MTT Assay, EdU Assay, Flow Cytometry, Migration, Transwell Assay, Over Expression, Plasmid Preparation